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Investigación y Patentes

EP22156902
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Analytical research
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Composition studies of products
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Characteristics and evaluation of functionality of the NL-BPC-157 product
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Characterization and evaluation of functionality of the NL-Epithalon product
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Characterization and evaluation of functionality of the NL-GHK-CU product
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Characteristics and evaluation of functionality of the NL-Cortagen product
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Characteristics and evaluation of the functionality of the NL-Selank product
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Characteristics and evaluation of functionality of the NL-Semax product
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Chromatography and MS spectrometry of basic peptides
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Chromatography and MS spectrometry of NL-peptides
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NL-PEPTIDES - Your new, better, patented peptides.

The tables below show a comparison of NL-peptides with regular peptides. Comparative studies were conducted in an environment reflecting the digestive system, as well as during the storage of the peptides themselves and in dissolved form (vials).

Conclusion: NL-PEPTIDES are much more stable than regular peptides and are therefore suitable for use in capsules and oral pharmaceutical compositions.

2.1. Analyses of basic peptides and NL-peptides in an environment resembling the stomach pH3 environment.

NL-BPC-157 VS BPC-157

NL-GHK-CU VS GHK-CU

NL-EPITHALON VS EPITHALON

NL-SEMAX VS SEMAX

NL-SELANK VS SELANK

NL-CORTAGEN VS CORTAGEN

2.1. Analyses of basic peptides and NL-peptides in an environment resembling the stomach environment at pH5.5.

NL-BPC-157 VS BPC-157

NL-GHK-CU VS GHK-CU

NL-EPITHALON VS EPITHALON

NL-SEMAX VS SEMAX

NL-SELANK VS SELANK

NL-CORTAGEN VS CORTAGEN

2.2. Stability of peptide lyophilisates during storage.

Lyophilized samples sealed in airtight vials were stored for 365 days at 35°C and standard humidity. Analyses were performed by opening successive vials at 1-month intervals.

NL-BPC-157 VS BPC-157

NL-GHK-CU VS GHK-CU

NL-EPITHALON VS EPITHALON

NL-SEMAX VS SEMAX

NL-SELANK VS SELANK

NL-CORTAGEN VS CORTAGEN

2.3. Stability of aqueous solutions during storage.

The stability of the structure was performed by analyzing peptide solutions prepared as follows: Peptide solutions were prepared at a concentration of about 1 mg/ml in demineralized water (pH about 5.6). The solutions prepared in this way were stored in controlled conditions at a temperature of 40 °C and humidity of 55% and the determinations were performed at regular time intervals.

NL-BPC-157 VS BPC-157

NL-GHK-CU VS GHK-CU

NL-EPITHALON VS EPITHALON

NL-SEMAX VS SEMAX

NL-SELANK VS SELANK

NL-CORTAGEN VS CORTAGEN

NL-PEPTIDES-DELIVERY TM - Your new patented double capsule technology.

Source: EP22156902 and P22156902

Results of blood NL-peptide concentration tests

The first three example graphs show peptide peaks from appropriately prepared blood samples, tested after 120 min., respectively, for: NL-GHK-CU, NL-EPITHALON and NL-BPC-157 administered in our patented NL-PEPTIDES-DELIVERY TM delivery technology, and for regular peptides GHK-CU, EPITHALON, ARG-BPC administered in regular capsules. The next three graphs show the distribution of peptide concentration in the tested blood in the time range of 30-360 min, converted to pg/ml units.

Conclusion: Thanks to our dual encapsulation technology, the use of peptides in oral form has become possible. In the graphs below we can see proof of the effect of our technology.

GHK-CU-HPLC wykres
EPITHALON-HPLC wykres
BPC-HPLC wykres

BIOTECHNOLOGICAL RESEARCH - how do our products work?

Source: Characterization and evaluation of product functionality NL-GHK-CU, NL-EPITHALON, NL-BPC-157, NL-CORTAGEN, NL- SEMAX, NL-SELANK.

For example: We have conducted studies such as: Evaluation of the effect of the in vitro digestion process on active substances contained in the products, along with the evaluation of the bioavailability of active ingredients through the intestinal-blood barrier. And: Evaluation of the rate of release of active substances (peptides) from the products in the digestive tract, as well as determining the dosage and many others.

Conclusion: Our NL-peptides penetrate the intestine in their entirety and easily cross the intestinal-blood barrier. In simple words, they work and their aminogram remains unchanged.

NL-BPC-157
NL-EPITHALON
NL-GHK-CU

PRODCT COMPOSITION TESTS - WHAT AM I BUYING?

Source: Product composition research.

The tested samples were applied in the amount of 15ul to the BioBasic 8 Thermo Scientific column. During the analysis using the linear gradient method in H20 and Acetonitrile solutions with the addition of 0.1% trifluoroacetic acid, peaks of the searched substances appeared. Their concentrations after comparison to the reference chromatogram are presented in the tables. The height of the measurement error is +- 0.5mcg.

Conclusion: Our products contain compositions consistent with the declared content.

NL-BPC-157
NL-GHK-CU
NL-EPITHALON
NL-SEMAX
NL-SELANK
NL-CORTAGEN

ANALYTICAL TESTS - AM I BUYING SAFE PRODUCTS?

Source: ANALYTICAL Research

The following tests were performed: presence of coronobacter, microbiology, alkaloids, presence of heavy metals, presence of ethyl oxide.

Conclusion: Our products are safe.

CORONOBACTER
MICROBIOLOGY
ALKALOIDS
HEAVY METALS
ETHYL OXIDE
RESEARCH REPORT

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